Molecular characterization of coriander (Coriandrum sativum L.) genotypes using random amplified polymorphic DNA (RAPD) markers

Abstract

Coriander (Coriandrum sativum L.) is an important seed spice crop grown in india and throughtout the world. Twenty–three genotypes of coriander were selected with an aim to find out genetic variability using random amplified polymorphic DNA (RAPD) marker. A total of 72 amplified bands were obtained using 14 RAPD primers out of which 48 were polymorphic. RAPD analysis showed 64.34% of polymorphism. The total number of amplified bands varied between 3 (primer S-34) and 11 (primer OPB-06) with an average of 7 bands per primer. The overall size of PCR amplified products ranged between 300 bp to 3000 bp. The average PIC was 0.229 ranging from 0.041 to 0.695. 3 unique bands were detected in 3 genotypes with 3 RAPD primers. The size of these unique bands ranged from 300 to 1400 bp. The Jaccard’s similarity coefficient values ranged from 0.63 to 0.93 with an average of 0.78. The dendrogram generated through UPGMA clustering method, genotypes divided into 2 main clusters. Cluster I is a major cluster including 22 genotypes and it could be further divided into several subclusters respectively. Cluster II included single genotype only. Hence, the overall grouping pattern of cluster analysis based on RAPD showed polymorphism among most of the genotypes.