Molecular characterization of garlic (Allium sativum L.) genotypes differ in total soluble solid content

Abstract

As garlic (Allium sativum L.) as an important role as medicinal and spices, quality of garlic plays major contribution in the fulfilling of its role. Now a day use of molecular markers for the evaluation of molecular diversity is receiving much attention than morphological characterization. Total 15 RAPD primers generated 84 bands in which all 84 bands were polymorphic having 82 shared and 2 unique bands with an average of 5.6 bands and 100% polymorphism per primer. Eighteen ISSR primers engendered 87 bands in which all 87 bands were polymorphic with 84 shared and 3 unique bands having 100% polymorphism with an average of 4.83 bands per primers. Total 12 SSR primers generated the 13 fragments in which all bands were polymorphic shared except primer SSR-13, which gave only one monomorphic band with 91.66% polymorphism with an average of 1 band per primer. An average polymorphism information content (PIC) value for RAPD primer, ISSR primer and SSR primer were 0.77, 0.69 and 0.04, respectively. Primer indices for RAPD, ISSR and SSR were 4.35, 3.52 and 0.08 respectively. The similarity coefficient of clusters analysis was ranged from 21 to 49 % for RAPD, 44 to 60% for ISSR and 49 to 75% for SSR.