In vitro cultural study of black spot of rose caused by Diplocarpon rosea against different solid media

Abstract

Black spot, the Diplocarpon rosae is a rose-specific fungal disease. It is caused by a common fungus, which thrives in humid, warm and wet conditions. This is prevalent and occurs across the world, and is the most serious rose disease in nearly all Rose species and cultivars. The fungus infects the leaves during the spring and reduces the vigor of the plant during the growing season. In-vitro cultural studies of Diplocarpon rosea were carried out of this five different solid media i.e. Sabouraud agar (SA), Potato dextrose agar (PDA), Czapek dox agar (CA),Corn meal agar (CMA), and Nutrient media (NA) of this PDA was recorded the maximum growth followed by Czapek dox agar (CA), and sabouraud agar (SA). where as nutrient agar recorded the minimum growth (NA).