Screening and in-vitro comparative evaluation of different isolates of Sclerotinia sclerotiorum under five selective media

Abstract

The aim was to find out best media to support the maximum mycelial growth of Sclerotinia sclerotiorum, with keeping this view in mind present experiment was conducted at Department of Plant Pathology, College of Agriculture, RVSKVV, and Gwalior Madhya. Pradesh. The five selected culture media were tested viz., Potato dextrose agar, Glucose sodium chloride agar, Beetroot dextrose agar, Richard’s agar, and Carrot dextrose agar, Corn meal agar, were evaluated against seven Sclerotinia sclerotiorum isolate of grid zone of M.P. The radial fungal growth recorded from 7th days after inoculation. The experiment was laid out in Complete Randomize Block Design with three replications. The maximum growth of all the isolates at 7 days after inoculation was recorded. In Glucose agar media, in which the maximum growth was recorded in Morena isolates (84.67 mm) followed by Guna (79.67 mm), Bhind (78.00 mm), Gwalior (77.67 mm), Sheopur (68.33 mm) and Ashoknagar (67.33 mm), while minimum growth was recorded in Shivpuri (64.00mm) as for as the mean performance of all the isolate concerned against the five selective media. The glucose media support the maximum growth pf Sclerotinia sclerotiorum. The average growth of pathogen was also compared on different media, the results revels that the average maximum growth was recorded in Morena isolate, while minimum growth in Shivpuri.