Effect of copper stress on biochemical changes in Philosamia ricini’s gland

Abstract

The purpose of the study was to analyze the effect of Copper sulphate stress on total protein, protease, and level of free amino acids in silkworm. Enzymatic activity of alanine amino transferase and aspartate amino transferase in silkworm were also assed. For this study collected eri silkworm eggs from Regional Sericulture Research Station, Hyderabad, Andhra Pradesh (Central Silk Board). These eggs were hatched after seven days at 26℃. The larvae at early stage were fed with the chopped green variety of castor leaves (Ricinus communis). After the larvae enter to the second instar, it fed with Copper sulphate treated castor leaves at different concentration of 5, 10, and 15ppm with control. During the time of experiments also analyze the length and weight of the larvae. At 15ppm Copper sulphate treatment both third and fourth instars maximum length (2.7 and 3.8cm), weight (0.29 and 0.79gm), total protein (65.11±0.08 and 58.86±0.09 mg/gm), free amino acid third instar 15ppm (13.48±0.08µg/ml), In fourth instar highest on 5ppm (14.97±0.05 µg/ml) of the larvae observed and minimum was observed at Copper sulphate of 10ppm concentration. In this study also analyzed Protease activity, AAT and ALAT was highest at 10 ppm concentration.