Qualitative and quantitative analysis of phytochemicals by GC-MS and antioxidant activity of Catharanthus roseus (L.) G. Don

Abstract

The present study was framed to evaluate bioactive phytochemical, antimicrobial and antioxidant properties of the sequentially extracts prepared from leaves of Catharanthus roseus in five different solvents (Petroleum ether, Chloroform, ethanol, methanol and aqueous). Antioxidant potential of the extracts was evaluated by both enzymatic assays (Superoxide dismutase, Catalase, Glutathione peroxidase) and non-enzymatic assays (DPPH, ABTS). Lowest IC50 values were shown by methanolic extracts followed by ethanol and aqueous extracts. The antioxidant activity of enzymes viz. catalase, glutathione peroxidase and superoxide dismutase were found to be satisfactory. The antioxidant activity of the extracts exhibited positive correlation with the total phenolic and flavonoid content of the extracts. The chemical composition of methanolic extract was also analysed by GC-MS. Seven phytochemical were predominantly identified in the methanolic extract by GCMS.