Development, validation and application of RP-HPLC method for estimation of Xymenynic acid in Santalum album seeds extract

Abstract

A simple, reproducible, feasible and innovative reversed-phase high performance liquid chromatographic method was developed and validated for the quantitative determination of Xymenynic acid present in Santalum album L. seeds extract. An extraordinary increase in the demand for skin lightning herbal formulations in the last two decades, and a need has been handled for ensuring quality, safety, and efficacy of herbal skin lightning drugs. High Performance Liquid chromatography with a photo diode array detector at a wavelength of 229 nm using a reversed-phase Zorbax stationary phase has been employed in this study. Isocratic elution is employed using a mixture of methanol- water (60:40v/v). This new method is validated for assay determination of Santalum album L. seeds extract, which include accuracy, precision, specificity, linearity, and range. This method expressions enough selectivity, sensitivity, accuracy, precision, and linearity range to satisfy International Conference of Harmonization regulatory requirements. A precise, accurate and reproducible HPLC method has been validated for analysis of xymenynic acid in S. album seed extract.