Standardization of ova Ark: An effort to standardise formulation from grandma’s pouch

Abstract

Ova ark is a traditional home remedy, used to get relief from routine gastric problems, in Maharashtra. Although Ajjowan is one of the main constituent, several other plants, Saindhava and asafoetida are added in the formulation which makes it more efficacious. The current study aims at standardization of the same formulation; using multidisciplinary approach.All the ingredients were first dried pulverized and the extract was prepared by steam distillation. This was repeated several times with variations in the amount of ingredients, to achieve perfect taste, aroma. Proximate analysis of the formulation was carried out and the formulation was further evaluated using TLC fingerprinting, GC and GC-MS Fingerprinting. Moreover MIC Assay and AAS were also carried out. GC-MS analysis of formulation confirms presence of Thymol and other Terpenoids, which is the active constituent of Ajjowan and other plants used in the preparation respectively. Formulation showed antimicrobial activity against Escherichia coli and Staphylococcus aureus. Iron and copper were found to be present by atomic absorption spectrophotometry. In conclusion, this study provides a standardized protocol for preparation and quality control parameters for the Ova ark formulations using conventional and sophisticated approach.