Challenges in micropropagation of Bael [Aegle marmelos (L.) Corr.] from leaf Disk Explants and methods for its adoption amongst progressive farmers and farm women

Abstract

The present study was performed to define tissue culture techniques for micropropagation of bael cvs. through leaf disk explants from 25 days old in vitro grown plantlets on Knop’s medium supplemented with 0.5 mgl-1 BAP. Leaf disk explants were cultured on various concentrations of growth regulators (BAP, KIN and NAA). Knop’s medium supplemented with 1.0 mgl-1 BAP, 0.5 mgl-1 KIN and 0.5 mgl-1 NAA produced maximum percentage of shoot regeneration (46.66%) and maximum per cent multiple shoot (33.33%) was found in cv. Mirzapuri on Knop’s medium supplemented with 1.0 mgl-1 BAP+0.5 mgl-1 KIN +0.5 mgl-1NAA. Maximum mean number of shoots per explants (1.20) was recorded in cv. Mirzapuri at 1.0 mgl-1 BAP+0.5 mgl-1 KIN+0.5 mgl-1NAA whereas maximum shoot length (0.95cm) was observed in cv. Mirzapuri on Knop’s medium supplemented with 1.0mgl-1 BAP+0.5mgl-1KIN+0.5mgl-1NAA.