Molecular characterization of Indian bean (Lablab purpureus L.) genotypes

Abstract

Molecular diversity of 20 Indian bean genotypes analyzed using molecular markers viz., Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeats (ISSR) and Simple Sequence Repeats (SSR). Indian bean genotypes were collected from Vegetable research station, Junagadh agricultural university, Junagadh.13 RAPD, 10 ISSR and 10 SSR primers produced total 78, 61 and 32 bands, respectively. RAPD and ISSR primers share 100% polymorphism, while SSR primer shares 98.33% polymorphism. Mean polymorphic content value highest for RAPD (0.72) and ISSR (0.71) as compared to SSR (0.43). SSR markers exhibit wide range of similarity (0.57-0.96) compared to RAPD and ISSR markers. SSR shows low primer index as compared to RAPD and ISSR primer. In Mental test, combined analysis of RAPD, ISSR and SSR shows r = 0.80 (good fit). Based on molecular data in the present study it can be concluded that the molecular markers could be a better tool for studying the genetic diversity.