Studies on the effect of different concentrations of kinetin on callus induction of dragon fruit plant (Hylocereus undatus)

Abstract

Dragon fruit (Hylocereus undatus) belong to family Cactacea, originated in Vietnam requires dry, tropical or subtropical climates and annual rainfall of 20-50". The plant bears high nutritional and pharmaceutical qualities so it can be a great option to the farmer in tropical region to cultivate the crop. To provide tissue culture technology can produce high quality and large scale plantlets. The present investigation was carried to optimize the concentrations of kinetin for induction callus in dragon fruit (Hylocereus undatus). The result reveled that Maximum fresh weight was produced in media supplemented with 2.5mg/l Kinetin and 0.01 mg/l constant NAA (T2). Whereas, least callus production was observed in T1 (1.0mg/l) Kinetin and 0,01mg/l NAA. At the concentration of kinetin (2.5 mg/l kinetin), the 15 days required after inoculation for callus initiation, the fresh weight observed 2.8 gm and the diameter of the callus was 15.86 mm which was recorded highest among all in Hylocereus undatus.