Detection of seed borne myco-flora associated with cowpea (<em>Vigna unguiculata</em> L. Walp)

Abstract

The seed borne myco-flora of cowpea cv. Phule Vithai was examined by blotter method, agar plate method, deep freezing, 2-4 D method and Test tube water agar seedling symptom test as recommended by ISTA. A total of four species of fungi viz. Alternaria alternata, Aspergillus flavus, Aspergillus niger, Fusarium oxysporum, Penicillium spp. and Fusarium moniliforme were reported. Amongst the methods used for detection of seed borne fungi, the standard blotter paper method is more effective followed by agar plate method, 2, 4-D method, deep freeze blotter paper method and test tube water agar seedling symptom test.