Analytical characterization and microbial studies of muscle lipid of Indian threadfin (Polynemus indicus) of the Bay of Bengal

Abstract

Lipid was extracted from the muscle of Indian threadfin (Polynemus indicus) by solvent extraction method and then purified. Various physico-chemical properties of the lipid sample including cholesterol content were determined and compared with other standard fats and oils. Fatty acid composition of the lipid sample was investigated by Thin Layer Chromatographic (TLC) examination. Gas Liquid Chromatographic (GLC) examination was performed by the methyl esters mixture prepared from the lipid sample for the quantification of fatty acids. The lipid containing selected muscle was analyzed quantitatively for the determination of percentages of protein and mineral (N, P, K, Ca, and Na). The muscle was also analyzed for determining the amount of several metals by using Atomic Absorption Spectrophotometric method. Microbial activities (bacterial activity against nine bacteria and fungal activity against seven fungi) of the lipid were studied by standard procedures.