Isolation, physico-chemical characterization andrnmicrobial studies of liver lipid of sting ray fish (Dasyatisrnsephen) of the Bay of Bengal

Abstract

Lipid was extracted from the liver of Sting Ray (Dasyatis sephen) by solvent extraction method and thenrnpurified by suitable conventional chromatographic method. Various physical and chemical characteristicsrnof the lipid sample were determined by standard methods and compared with those of standard fats orrnoils. Fatty acid composition of the lipid sample was investigated by Thin Layer Chromatographic (TLC)rnexamination. The liver lipid of Sting Ray was found to contain palmitic acid, stearic acid and oleic acidrnwith some other unknown fatty acids. Antimicrobial activity (antibacterial activity against four bacteriarnand antifungal activity against four fungi) of the lipid was tested by standard methods. The lipidrncontaining liver of Sting Ray was analyzed quantitatively for the determination of percentages of proteinrnand mineral (N, P, K, Ca) contents by modified Kjeldahl method.