Chromatographic fingerprint analysis of Burmese grape (Baccaurea ramiflora Lour.) by HPTLC technique

Abstract

The objective of the present study is to investigate the phytochemistry along with TLC and establish the High Performance Thin Layer Chromatography (HPTLC) fingerprint profile for the first time of the three different extracts viz. acetone, methanol and aqueous extracts of the bark of Baccaurea ramiflora, the wild edible plant used by the tribal people of Meghalaya, India. Camag HPTLC system equipped with TLC Linomat V applicator, Camag TLC scanner III and win CATS software were used. Acetone, Methanol and Aqueous extracts of the bark of Baccaurea ramiflora were developed in suitable mobile system using standard procedures and scanned under UV at 254 nm and 366 nm. Phytochemical screening showed the presence of phytosterols, gums and mucilage. The HPTLC fingerprint scanned at 254 nm revealed 11 peaks with Rf value in the range of 0.07 to 0.92 for acetone extract, 7 peaks with Rf value 0.08 to 0.77 for methanol extract and 6 peaks with Rf value 0.07 to 0.62 for aqueous extract with mobile system I, similarly 11 peaks with Rf value in the range of 0.07 to 0.87 for acetone extract, 10 peaks with Rf value 0.09 to 0.88 for methanol extract and 8 peaks with Rf value 0.07 to 0.58 for aqueous extract with mobile system II. Phytochemical screening, TLC and HPTLC analysis of Baccaurea ramiflora can provide standard reference for the proper identification/authentication and quality control of the drug and will be helpful in differentiating the species.