Finger printing analysis of the flavonoids from Holoptelea integrifolia (Roxb.) planch leaves using HPTLC analysis

Abstract

Objective The present study was conducted to identify the flavonoids from petroleum ether and methanol extracts of medicinal andeconomicallyuseful leaves of Holopteleaintegrifolia (Roxb.) Planch using High Perform ance Thin Layer Chromatography (HPTLC) technique. Methods Preliminary phytochemical screening was done and HPTLC studies were carried out. CAMAG HPTLC system equipped with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungsten lamp. Results Preliminary phytochemical screening of petroleum extract of Holoptelea integrifolia showed the presence of steroids, terpenoids, alkaloids, glycosides, flavonoids, proteins, tannins and carbohydrates, while methanolic extract of Holoptelea integrifolia showed the presence of steroids, alkaloids, flavonoids, proteins and carbohydrates. HPTLC fingerPrinting of flavonoids of petr oleum ether extract of leaf revealed five polyvalent phytoconstituents (5 peaks) and corresponding ascen ding order of Rf values in the range of 0.09 to 0.84. While methanol extract of leaf showed eight polyvale nt phytoconstituents (8 peaks) and corresponding ascending order of Rf values in the range of 0.08 to 0.81. Conclusions With the above Rf values and preliminary phytochemical analysis we have concluded the presence of flavonoids in both the extracts.