Optimization of culture conditions using response surface methodology for synergism production of cellulase, xylanase and pectinase by Rhizopus delemar F2 under solid state fermentation

Abstract

In the present study Response surface methodology (RSM) was used to investigate the combined effect of relevant process variables to maximize the production of multiple carbohydrase in solid state fermentation by Rhizopus delemar F2 using environmental waste i.e. apple pomace a major horticultural waste of Himachal Pradesh. The process variables include temperature (25, 30, and 35°C); moisture (1:2-1:5) and incubation days (4-10). A 20 factorial central composite design (CCD) using response surface methodology (RSM) was employed to obtain interaction between the process variables and optimizing these variables. Total 20 experiments were carried out in shake flask and a three dimensional response surface was generated to determine the effect of process variables on enzymes production. The optimal calculated values of tested variables for maximal production of enzymes were: temperature 30°C, moisture ratio of 1:3:5 and 7 days of incubation with 10.40 U/gds of cellulase, 126.80 U/gds and 31.20 U/gds.