HPLC analysis of saponins in Achyranthes aspera and Cissus quadrangularis

Abstract

Reversed phase high performance liquid chromatography (RP-HPLC) with UV detector was done for the analysis of four saponins of Achyranthes aspera and Cissus quadrangularis. The separation of filtered plant extracts as well as a mixture of authentic standard saponin samples of Gitogenin, Diosgenin, Hecogenin, Kryptogenin was done within 25 min. Running conditions included: injection volume 15µl; mobile phase: acetonitrile: water (40:60) running time 25 min., flow rate 1 ml/min; and detection at 203nm. Identifications of specific saponins are made by comparing their retention times with those of the standards. Three saponins in Achyranthes aspera namely Gitogenin, Diosgenin, Kryptogenin and two saponins namely Diosgenin, Kryptogenin in Cissus quadrangularis were detected by HPLC.