Bio-assay guided isolation of lead bioactive molecules from Spirogyra rhizopus C-C. Jao

Abstract

Scientific and technological advancement which propelled the scientists in drug discovery research to design as well as synthesize novel drug to treat multidrug resistant microorganisms. Spirogyra alga is the source of novel bioactive phytochemicals and contains carbohydrates, proteins and fats. Among the different crude extracts prepared from Spirogyra rhizopus C.-C. Jao, Chloroform crude extract showed promising activity against four bacterial strains Salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The present study aimed to investigate the bioassay guided isolation of lead bioactive molecules from active chloroform crude extract and evaluate their antibacterial potential against S. typhimurium, E. coli, S. aureus and P. aeruginosa by disc diffusion method, column chromatography isolation of crude extract leads to SP-1, SP-2, SP-3 and SP-4 bioactive molecules and structural elucidation was done with the help of 1H-NMR, 13C-NMR, IR and DEPT spectral studies.