Physicochemical and HPTLC analysis of Pippalimula (Root of Piper longum Linn.)

Abstract

Background: Pippalimula (root of Piper longum Linn.) is a well-known herb used as single drug or in compound formulations for various disorders. Aim: The study was planned to carry out the Physicochemical and HPTLC analysis of Pippalimula. Material and Methods: Fine powder of Pippalimula and alcoholic extract were obtained and subjected to phytochemical analysis and chromatographic study. Result: Physico chemical analysis of the root was carried out and found similar to reported API standard limits. HPTLC analysis was performed with Toluene: Ethyle acetate: acetic acid (7:2:1) v/v. Which showed 3 spots on 254 nm. HPTLC analysis of root of Piper longum Linn.can provide standard analysis with selected solvent system and can be used as a reference for the authentication and quality control of the drug.