articleTop 1% cited

Advantages of Single-Nucleus over Single-Cell RNA Sequencing of Adult Kidney: Rare Cell Types and Novel Cell States Revealed in Fibrosis

Journal of the American Society of Nephrology · 2018 · Vol. 30(1) · pp. 23–32

Hao WuYuhei KiritaErinn L. DonnellyBenjamin D. Humphreys✉(Washington University in St. Louis)

Abstract

snRNA-seq achieves comparable gene detection to scRNA-seq in adult kidney, and it also has substantial advantages, including reduced dissociation bias, compatibility with frozen samples, elimination of dissociation-induced transcriptional stress responses, and successful performance on inflamed fibrotic kidney.

Single-cell and spatial transcriptomics Renal and related cancers Renal and Vascular PathologiesBiologyRNACellTranscriptomeCell biologyCell typeSmall nuclear RNAGeneKidneyMolecular biology

MeSH terms

AdultAnimalsCell NucleusCells, CulturedDietary ProteinsDisease Models, AnimalFibrosisGene Expression RegulationHumansKidney DiseasesMice, Inbred C57BLRNA, Small NuclearSensitivity and SpecificitySequence Analysis, RNAGene Expression Profiling

Citations

770

FWCI

23.02

field-weighted impact

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100%

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References

Fabrication of microfluidic systems in poly(dimethylsiloxane)