Isolation and Biological evaluation of Endophytic Fungus from Ziziphus nummularia

Abstract

The aim of the current research work was to analyzed Endophytic fungi and there enzymatic activity. The research work was carried out in the Microbiology Lab, University of Haripur, during May 2015 to September 2015.The plant was collected and fungi were isolated by inoculating the leaf sections on different Medias. Six fungal species were isolated and identified on Sabouraud Dextrose agar medium. The results indicate highest colonization frequency at Sabouraud Dextrose Agar media that is 83%. Frequency of colonization was 76%, 26% and 23% on Malt Extract Agar, Potato Dextrose Agar and Czapak Dox Agar respectively. Identification was done by comparing with available literature and by microscopy. The study on this plant revealed that plant contains endophytic fungi that have ability to produce amylase, protease and lipase enzymes, although better activity for production of amylase enzyme was observed.