Isolation of acid and pepsin soluble collagens from the skin of Indian mackerel Rastrelliger kanagurta (Cuvier, 1817)

Abstract

The objective of this study was to isolation and compare the acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from the skins of Indian mackerel. Collagen was extracted from the skin of Rastrelliger kanagurta, the fish from marine water. Acid soluble collagen (ASC) and Pepsin soluble collagen (PSC) were extracted from mackerel skin. The yield of ASC and PSC from mackerel skin was recorded as 9.32% and 7.68% respectively; higher yield was recorded in ASC method. It was found that the major constituents of both ASC and PSC consisted of alpha chains (ɑ1, ɑ2) and beta (β) chains. Molecular weight pattern from mackerel skin collagen is shown in various bands, these bands with molecular weight in between 135 kDa to 180 kDa in ASC and PSC pattern. The proximate composition of collagen extracted from mackerel skin i.e. moisture, crude protein, fat and ash content in collagen was recorded as 9.17%, 87.74%, 1.23% and 1.86% respectively.