Extraction and estimation of chlorophyll content of seed treated lentil crop using DMSO and acetone

Abstract

Chlorophyll is content responsible for green pigment and structurally similar to porphyrin pigments such as heme having same metabolic pathway. In present study Chlorophyll content were extracted from lentil leaves treated with bio control agent and fungicide by using dimethyl sulfoxide (DMSO) and acetone. The prepared samples were exposed to a range of light having different wavelength and analyzed using a spectrophotometer. Concentration of chlorophyll a and b was calculated using Arnon method. Chlorophyll content was higher in leaves treated with bio control agent and fungicide over control.