Optimization of carotenoid pigment production by yeast Sporobolomyces sp. using response surface methodology

Abstract

The Response Surface Methodology (RSM) technique was performed to increase the carotenoid pigment production of Sporobolomyces sp. The Central composite design involving the variables pH (A), fermentation time (B) and incubation temperature(C) for the production of carotenoid pigment by Sporobolomyces sp was standardized. Data analyzed using a second order polynomial equation resulted in the optimized process condition for pigment production was 120 h growth at 25 °C in a Yeast extract dextrose broth of pH 5.0. Using this experimental design, the total carotenoid production yield increased from 674.34 to 990.87 µg g-1. The model showed that the value of R2 0.9986 was high and p- value of interaction of variance was