Isolation of compound (CA-01) from the bark of Careya arborea by using column chromatography

Abstract

Careya arborea Roxb, commonly known as wild guava. Belonging to the family Lecythidaceae is known as Padmaka in Ayurveda. It is used as astringent, demulcent, antitumour, antipyretic and antipruritic, hepatoprotective, antimicrobial, antioxidant, CNS activity, antileishmanial, anticoagulant, analgesic, cytotoxic activity. The authenticated barks were procured from Sri Venkateshwara University, Tirupathi. The coarse powder of bark of Careya arborea subjected for extraction process by using soxhlet apparatus by using successive solvents. The methanol solvent produces highest extractive yield. Therefore the methanolic extract of bark was used for optimization of solvent systems for TLC studies. The mobile phase selected was n-butanol: glacial acetic acid: water (4:1:5) showed best pattern of band separation. So that the methanolic extract was subjected to column chromatography. Therefore the extract packed into column and eluted with hexane: acetone to obtain 11 fractions. The 10th fraction showed the three bands with similar chromatographic pattern by TLC identity tests. This fraction was subjected for preparative column for isolation and purification of compound. Purity of the isolated compound was determined by HPLC analysis. Structural determination/ characterization was done by UV, IR spectroscopic analysis.