Instant genomic DNA extraction method from safflower leaf for PCR Analysis

Abstract

In spite of the possibility of analysing large samples and the rapidity of Polymerase Chain Reaction (PCR) technology, the usual protocols for DNA plant extraction remain time-consuming, slow and even hazardous. To lessen labor, time or cost of DNA extraction, a simple and instant method for genomic DNA extraction from leaf tissue of Safflower (Carthamus tinctorius) leaf is established. Small quantity of tissue materials (typically 3-5 mg) was ground in a centrifuge tube using plastic pestles in extraction solution. Extracted DNA was suitable for PCR analysis, without centrifugation. The feasibility of this method was confirmed by testing molecular markers and transgene detection. This method requires less than 1 mg of plant tissue stored frozen or used fresh and is useful for molecular marker, transgene detection, and other experiments.