A modified and efficient CTAB genomic DNA extraction method from maize leaf for PCR analysis

Abstract

High throughput DNA isolation process is time consuming and laborious steps in advanced molecular genetic analysis process viz. gene mapping, DNA fingerprinting, marker assisted selection and purity analysis etc. In present study described a simple, cost-effective modified CTAB-DNA isolation from the leaf of maize, excluding liquid nitrogen and 2-mercaptoethanol that can be performed in a laboratory with basic facilities. Presence of non-cellulosic components and starch in cell-wall of leaves reduce the quality and also hinders the DNA isolation process. This method is simple, rapid, low cost, and reliable for PCR analysis.