Antioxidant potential of pod coats extracts of cluster bean and their efficacy in stabilizing soybean oil

Abstract

Present investigation was conducted to determine phenolic composition and antioxidant activity of pod coat extracts of cluster bean and their effect on tocopherols and carotenoids content of crude soybean oil. Pod coats were extracted with solvents of varying polarity (acetone, ethyl acetate and chloroform). Extract yield as well as concentration of different phytoconstituents varied in different solvent. Highest yield was observed in acetone extract (5.44%). Total phenolics, flavonoids and tannins content were also highest in acetone extract (24.18±0.12 mg GAEg-1, 10.77±0.09 mg CAEg-1 and 2.48±0.03 mg TAEg-1). Tocopherols were highest in ethyl acetate extract (7.10±0.04 mg/g). Acetone extract showed highest DPPH free radical scavenging activity i.e. 77.43%. Tocopherols (33.04 mg/100g of oil) and carotenoids content (20.48 mg/kg of oil) were highest in the soybean oil sample stabilised with acetone extract (2000ppm) of pod coat of cluster bean at the end of incubation period.