Anthelmintic activity of abutilon indicum leaf extract on sheep tapeworm Moniezia expansa In vitro

Abstract

Abutilon indicum methanol leaf extract was tested against sheep tapeworm in in vitro condition with different doses of 25, 50 and 100 mg/ml concentration. Dose dependant cestodicidal activity of the extract was observed in the study with 100 mg/ml concentration and the time taken for the paralysis of the organism amounts to 66.3 ± 0.03 and death was recorded after 93.2±0.09 minutes No death was recorded even after 8 hours in the negative control (1%DMSO). The positive control was maintained with 10 mg/ml of Albendazole where the paralytic time and death time was recorded as 72.1 ± 0.13 and 93.5 ± 0.11 minutes. Biochemical analysis reveal the decreasing trend in the level of protein in different regions of the tapeworm, However, the changes observed in the level of carbohydrate and lipids were not significant. Light microscopic observation reveals the presence of sub-tegumental layer with circular and longitudinal muscles, presence of paranchymal cell along with glandular cells is also evident. Disintegration of sub-tegumental layer along with necrosis of paranchymal cells and glandular cells was observed in treated groups. GC-MS analysis of the leaf extract shows the presence of potential anthelmintic compounds eugenol and asarone. Separation and purification of potential anti-cestodal compounds will be a better natural alternative to control helmintic infections.