In vitro comparative evaluation of anti-inflammatory and thrombolytic activity of three Mikania species available in Bangladesh

Abstract

The present study was designed to investigate comparative anti-inflammatory and thrombolytic activity of the ethanolic extract of Mikania cordata (MC), Mikania micrantha (MM) and Mikania scandens (MS) (Family: Asteraceae). Phytochemical screening of these three species was also evaluated. Anti-inflammatory activity was performed using hypotonic solution induced hemolysis and heat induced hemolysis at various concentration and the result was expressed as % of inhibition of haemolysis. Ethanolic extract of MC, MM, MS and standard aspirin at 500µg/ml showed 80.943±0.64, 71.465±0.16, 60.351±0.40 and 92.894±0.30 % inhibition for heat induced haemolysis and 87.098±0.05, 86.242±0.45, 77.709±0.43 and 97.520±0.38% inhibition for hypotonic solution induced haemolysis, respectively. The data showed MC possessed strong anti-inflammatory activity compared to standard aspirin. The activity order was Aspirin>MC>MM>MS. In thrombolytic activity experiment % of clot lysis in MC, MM, MS and standard streptokinase were found to be 40.36±0.11, 48.44±0.34, 55.80±0.54 and 70.00±0.43 %, respectively, indicating moderate thrombolytic activity of the extracts compared to standard. The activity order was streptokinase>MS>MM>MC. The phytochemical screening showed the extracts contains alkaloids, terpenoids, tannins and cardiac glycosides. From the observation it was concluded that Mikania cordata is a good candidate for anti-inflammatory activity whereas Mikania scandens for thrombolytic potential.