Investigation of in-vitro Cytotoxicity and Thrombolytic activity of methanolic extract of Sida acuta (flower)

Abstract

The present study is to investigate in-vitro Cytotoxicity, Thrombolytic activity, of methanolic extract of Sida acuta (flower).Method: Methanol extract of Sida acuta (flower), was used to evaluate its cytotoxicity in Brine shrimp lethality bioassay where vincristine sulphate was used as standard drug. Thrombolytic effect of the extract was investigated in clot lysis experiment.. Result: In Brine shrimp lethality bioassay, LC50 value of the extract was 67.5μg/ml and vincristine sulphate served as the positive control showed LC50 value 14.55μg/ml. The extract exerted 24.786% lysis of the blood clot in thrombolytic activity test while 80.22%. And 7.5% lyses were obtained for positive control (streptokinase) and negative control respectively. Conclusion: Compared to vincristine sulphate, it is evident that the methanol extract of leaves of Sida acuta were cytotoxic. The extract again possessed considerable thrombolytic activity