ROS scavenging Mn₃O₄nanozymes for<i>in vivo</i>anti-inflammation

Abstract

Reactive oxygen species (ROS)-induced oxidative stress is linked to various diseases, including cardiovascular disease and cancer. Though highly efficient natural ROS scavenging enzymes have been evolved, they are sensitive to environmental conditions and hard to mass-produce. Therefore, enormous efforts have been devoted to developing artificial enzymes with ROS scavenging activities. Among them, ROS scavenging nanozymes have recently attracted great interest owing to their enhanced stability, multi-functionality, and tunable activity. It has been implicated that Mn-contained nanozymes would possess efficient ROS scavenging activities, however only a few such nanozymes have been reported. To fill this gap, herein we demonstrated that Mn₃O₄ nanoparticles (NPs) possessed multiple enzyme mimicking activities (<i>i.e.</i>, superoxide dismutase and catalase mimicking activities as well as hydroxyl radical scavenging activity). The Mn₃O₄ nanozymes therefore significantly scavenged superoxide radical as well as hydrogen peroxide and hydroxyl radical. Moreover, they were not only more stable than the corresponding natural enzymes but also superior to CeO₂ nanozymes in terms of ROS elimination. We showed that the Mn₃O₄ NPs not only exhibited excellent ROS removal efficacy <i>in vitro</i> but also effectively protected live mice from ROS-induced ear-inflammation <i>in vivo</i>. These results indicated that Mn₃O₄ nanozymes are promising therapeutic nanomedicine for treating ROS-related diseases.