In vitro antioxidant, antimicrobial properties and total phenolic contents of Citrus limetta collected from Sonapur, Assam, India

Abstract

The objective of the present work was to evaluate the antioxidant, antimicrobial activities and phenolic contents of juice of Citrus limetta from Sonapur, Assam, India. Antioxidant Assay was determined by DPPH free radical scavenging activity, Super oxide dismutase (SOD) and Nitric oxide radical scavenging activities. Total phenolic content was determined by using catechol as standard. MIC for two different microbes was obtained by spectrophotometry method. The extracted juice was used for various estimations to measure total antioxidant, super oxide dismutase and nitric oxide activity in-vitro and determined the antimicrobial potentials. The in vitro antioxidant activity of the extracted juice (at concentrations 12.5-100 µl/ml of methanol) was determined by DPPH free radial scavenging activity and found in the range of 56%-95.0% respectively. Super oxide dismutase and nitric oxide scavenging activities at 100 µl of juice extract were found to be 74% and 54.8%, respectively. Total phenolic content was found 0.007 mg/ml. Minimum inhibitory concentration (MIC) was found for 75% juice for E. coli and at 50% juice for P. aeruginosa. The results obtained in this research work clearly indicated a promising potential of Citrus intake for playing a protective role against oxidative stresses and can inhibit the growth of highly resistant and opportunistic bacteria.