Conventional and molecular detection of Salmonella species in backyard poultry of Odisha state in India

Abstract

A total 151 faecal samples were collected randomly from the backyard poultry birds from different rural areas of Odisha state. Out of 151 samples 20 samples were positive for presence of Salmonella spp. Multiplex polymerase chain reaction (PCR) technique was used in this study to differentiate different of serotypes using primers targeted to amplify phoP gene, Hin and H-li genes. No sample was positive for rfbs gene primer specific to Salmonella pullorum and Salmonella gallinarum. Prophylactic measures should be taken into account for the control of Salmonellosis in backyard poultry.