Antioxidant potential of Allium sativum, Cinnamomum zeylanicum and Azadirachta indica against free radicals and their antimicrobial activity against isolated microbes from diseased Tilapia

Abstract

Present study dealt with antioxidant activity, minerals profile and antimicrobial activity of Azadirachta indica, Allium sativum and Cinnamomum zeylanicum conducted in 2014. Total phenolic compound was higher in A. indica (279.64 ±17.34 mg GAE/100g) moderate in A. sativum (40.80 ± 2.91 mg) and lower in C. zeylanicum (11.66 ± 1.76 mg) similar in case of flavonoids content. Antioxidant activity was assessed in vitro using FRAP, DPPH, ABTS free radicals and copper and iron chelating activity, superoxide and hydroxyl scavenging. Results revealed plants extracts have strong antioxidant and free radical scavenging activity. In order to verify antimicrobial activity, Pseudomonas sp. and Aeromonas hyrophila were isolated from unhealthy tilapia. Ethanolic extract of A. indica showed maximum inhibition against both strains. A. sativum was moderate and C. zeylanicum least in action. It was concluded that test plants have strong antioxidant and antimicrobial activity and could be used in pharmaceutical formulation against various disorders.