Molecular diagnosis of naturally infection with Eimeria stiedae in domestic rabbits in Baghdad city-Iraq

Abstract

This study was designed for parasitological and molecular diagnosis of hepatic coccidiosis in rabbits. The ten hindered of local breed rabbits (Oryctolagus cuniculus) of both sexes were used. The animals were brought from the local markets in Baghdad city during the period from the beginning of January 2016 to 30th May 2016. The total infection rate was 19%, in which 18% males and 20% in females respectively with no significant difference at the level of (P>0.01). The high infection rate in young rabbits were (30%) and in adult (8%). The DNA amplification was confirmed by the electrophoresis analysis depending on DNA marker (976 bp). The results of this study confirmed high infection rate and more efficient monitoring of infection by using polymerase chain reaction as a the modern molecular diagnostic techniques of hepatic coccidiosis.