An attempt to prepare an effective killed vaccine with an adjuvant (green tea extract) against Salmonella typhimurium

Abstract

The present research was carried out to study the efficacy of green tea extract as a potent adjuvant mixed with killed Salmonella typhimurium (S.T.) vaccine to enhance its immunization capacity during the period from September 2014 to February 2015. Three groups of mice were immunized with the mixture of S.T. and green tea extract, S.T. alone, and phosphate buffered saline. The mixture group showed the best results in cell-mediated immunity; in which a significant difference (P< 0.005) in the skin thickness was detected (1.05±0.21 after 24 h and 1.35±0.162mm after 48 h and also higher records in the ELISA 1.342±0.650 nm and 1.114±0.018 nm after 35 and 53 days from the beginning of immunization, respectively. The results were verified by examination of spleen and liver histological sections. The present study suggests the effectiveness of the green tea extract as a potent adjuvant mixed with killed Salmonella typhimurium (S.T.) vaccine for the enhance the immunization of mice.