In vivo and In vitro biochemical estimation of primary metabolites from Helicteres isora: An important medicinal plant

Abstract

Helicteres isora is an important medicinal herb belonging to family Sterculiaceae. It is commonly known as ‘Marorphali’. Several important chemical compounds like Cucurbitacin B, isocucurbitacin B, tetratriacontanol, tetratriaecontanoic, acid, fridelin, lupeol, taraxerone, diosgenin, neolignans, helisterculins A and B, helisorin, tannins, triterpenoids, alkaloids, saponins, flavonoids, phlobatannins, anthaquinones are present in Helicteres isora. Plants provide biologically active metabolites and lead structures whose activities can be enhanced by manipulation through combinations with chemicals and synthetic chemistry. These metabolites can be exploited in the field of new drugs research and for the development of modified derivatives with enhanced activity and reduced toxicity.In present investigation, callus raised from leaf explant and different plant parts of Helicteres isora were evaluated for their biochemical estimation of primary metabolite quantitatively. Results showed that maximum callus was obtained on MS medium supplemented with a combination of BAP (1.0 mg/l) and NAA (1.0 mg/l). Variation in the content of primary metabolites have been found in different in vivo and in vitro samples of Helicteres isora. It further concludes that Helicteres isora serves as a rich source of primary metabolites which can be used as raw material in industries.