Phytochemical investigation and spectral studies of isolated flavonoid from ethanolic extract of whole plant Blumea lacera D.C.

Abstract

Blumea lacera D.C. (Asteraceae) is one of the common Rabi weeds of India. It is an annual herb, with a strong odor of turpentine. Indigenously in the Indian system of traditional medicine- Ayurveda it is used as bitter, astringent, acrid, thermogenic, errhine, anti-inflammatory, styptic, ophthalmic, digestive, anthelmintic, liver tonic, expectorant, febrifuge, antipyretic, diuretic, deobstruant, and stimulant. Phytochemical investigation on the aerial parts of Blumea lacera revealed to be quite rich in essentials oils contain Nagi camphor as its major constituent, I²-caryophyllene, I±-humulene and E-I²-farnesene, thymoquinol dimethyl ether and precocene. It also contains other volatile fraction of cineol, d-fenchone, and citral. In course of a systematic phytochemical studies of the Blumea lacera led us to isolate a flavonoid compound-I, (5-hydroxy-3’-methyl-3, 6, 7, 4’-tetramethoxyflavone) from the ethanolic extract of the whole plant. Isolated flavonoids from the ethanolic extract of Blumea lacera DC were separated and purified under column chromatography and analyzed by spectroscopy methods (UV, IR, 1H NMR, and Mass).