Evaluation of antiangiogenic property of Ocimum basilica ethanolic leaf extract by using duck embryo chorioallantoic membrane (cam) assay and its morphometric analysis

Abstract

Angiogenesis is the formation of new vessels that sprouts from endothelium, where there is endothelial cell migration, tube formation and proliferation. The Ocimum basilica, is an important herb under the Family Lamiaceae housing the largest number of medicinal species known for its therapeutic significance. The setups were composed of a vitamin A as positive control, 90% ethanol as negative control, O. Basilica ethanolic leaf extract in 1 mg/mL, 3 mg/mL and 5 mg/mL concentrations. The different concentrations were applied on the 8th day of incubation. The antiangiogenic effect of Ocimum basilica by taking the average number of branch points using CAM and was determined after two days. Results shows that angiogenesis was induced in the negative control while the inhibition of angiogenesis was significantly reduced with the O. basilica ethanolic leaf extracts. It also showed that the greater the dosage, the lesser the branched points observed. Thus, O. basilica ethanolic leaf extract might have a very promising antiangiogenic potential.