In vitro Membrane Stabilizing Activity, Total Phenolic Content, Cytotoxic, Thrombolytic and Antimicrobial Activities of Calliandra surinamensis (Wall.)

Abstract

Different extractives of leaf of Calliandra surinamensis were evaluated for antimicrobial activity along with membrane stabilizing, thrombolytic and cytotoxic activities. The total phenolic content was also determined and expressed in gallic acid equivalent. The membrane stabilizing activity was assessed by heat and hypotonic solution induced method. In the present studies, the crude methanolic extract (ME) of leaf of C. surinamensis demonstrated strong membrane stabilizing activity, whereas its chloroform (CSF) and pet ether (PESF) soluble fractions revealed moderate membrane stabilizing properties. In brine shrimp bioassay, the aqueous (AQSF) soluble fractions revealed the highest lethality. In antimicrobial screening, the choloroform (CSF) and aqueous (AQSF) soluble fraction demonstrated strong antimicrobial activity against 13 pathogenic microorganisms. The chloroform (CSF) soluble fraction exhibited the highest inhibition against microbial growth having zone of inhibition ranged from 13.0±0.82 mm to 18.3±1.25 mm and maximum zone of inhibition was found 18.3±1.25 mm against Salmonella typhi. In thrombolytic study, the chloroform (CSF) soluble fraction demonstrated mild thrombolytic activity in human blood specimen.