Screening of antibacterial activity and biochemical assay from haemolymph of cockroach Blatta orientalis (Linnaeus, 1758)

Abstract

The present study deals with the antibacterial activity of B. orientalis haemolymph against five bacterial strains namely, Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus aureus, Escherichia coli and Salmonella typhi. The results of the present study showed the maximum zone of inhibition against Escherichia coli, moderate effects were observed in Pseudomonas aeruginosa, Staphylococcus aureus and Proteus mirabilis, whereas the less effect was observed in Salmonella typhi. In SDS-PAGE analysis the prominent bands were observed at 18 kDa, 30 kDa and 66 kDa. The results of FTIR analysis revealed the peak ranges from3299 cm-1 to 546 cm-1. The FTIR spectral studies clearly indicate the presence of various functional groups such as Secondary sulphonamide, Secondary amide, Cyclopeptane, Vinyl halides, Aromatics compound, Triazoles, Thiophenes, Aldehyde group, Methylene compound, Sulphinic acid group, Bromo compounds which may be attributed to the antibacterial activity properties. The results of present study showed that the haemolymph of B. orientalis may have the potential antimicrobial activity.