Comparative HPTLC fingerprint profile of Illicium verum Hook.f. and Illicium griffithii Hook.f. & Thoms fruits

Abstract

To establish the fingerprint profile of two Illicium species namely Illicium verum Hook.f. and Illicium griffithii Hook.f. & Thoms using high performace thin layer chromatography (HPTLC) technique. Methods: CAMAG HPTLC system equipped with TLC autosampler 4 applicator, TLC scanner 3 and win CATS 1.4.4. software was used. n-hexane, chloroform and ethyl acetate extracts of the fruits were developed in suitable mobile phase using standard procedures and scanned under UV at 254 nm, 366 nm and under visible light. Results: The HPTLC fingerprinting of the n-hexane, chloroform and ethyl acetate extracts showed several peaks with different Rf values. Conclusion: HPTLC fingerprint profile of two Illicium species may be useful in differentiating the species and also helpful in the identification and authentication of these species.