Genetic variation of Rana parvaccola (Inger, Stuart and Iskandar, 2009) based on DNA microsatellites in West Sumatra

Abstract

Rana parvaccola is a new species of differentiated Rana chalconota complex in Sumatra. We have analyzed the genetic variation of 30 individuals R. parvaccola with Microsatellite DNA in West Sumatra. Five primers were used in the amplification process with PCR technique. The highest expected heterozygosity (He = 0.6533) was Padang population and the lowest (He = 0.2501) was Siberut population. There is a very high genetic differences between populations of R. parvaccola in West Sumatra (FST = 0,3452) with inbreeding coeffesient value FIS = -0,4152 and FIT = 0.0849. The value of gene flow between populations was very low (Nm = 2.0613).