Leaf essential oil composition, antimicrobial and cytotoxic activities of Cleistocalyx operculatus from Hetauda, Nepal

Abstract

The present study investigated the chemical composition and bioactivity of the leaf essential oil from Cleistocalyx operculatus (Roxb.) Merr. & L.M. Perry from Nepal. In this work, leaf essential oil of C. operculatus was obtained by hydrodistillation and analyzed by GC-MS. The major component of the leaf oil was myrcene (69.7%), followed by (E)-β-ocimene (12.24%), (Z)-β-ocimene (4.79%), and linalool (4.08%), with other minor constituents. Antimicrobial activities were determined using the microbroth dilution technique, and in-vitro cytotoxic activity against MCF-7, MDA-MB-231 and 5637 cells was tested using the MTT assay. C. operculatus oil has shown no antimicrobial or cytotoxic activity.