In vitro standardization of flowers of methanolic extract of Dendrobium normale Falc. for free radical scavenging activity

Abstract

Traditional remedies, often closely resembling or forming the basis for alternative remedies, may comprise primary healthcare or be integrated into the healthcare system. Numerous diseases are induced by free radicals via lipid peroxidation and protein peroxidation. It has been validated that a variety of herbal extracts have antioxidant activities to scavenge free radicals. Dendrobium normale Falc. Belongs to the family Orchidaceae; an ethnomedicinal plant. The aim of present work was to evaluate in vitro antioxidant potential of methanolic extract of D. normale by DPPH, hydroxyl and superoxide radical scavenging assays. The radical scavenging activity was found to be concentration dependent and increased with increased concentrations and produced maximum scavenging activity at a dose of 360 µg. The antioxidant potential could be due to the presence of flavonoids, alkaloids, triterpenoids, steroids and carbohydrates. Further, this can be confirmed by qualitative analysis.