Genetic variation among asang fish (Osteochilus vittatus Cyprinidae) populations using random amplified polymorphic DNA (RAPD) markers

Abstract

Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was applied to analyze the genetic variation among Osteochilus vittatus collected from three geographically distant location of West Sumatra Province. After initial PCR screening, four random oligodecamers viz OPA 07, OPA 09, OPA11 and OPA 18 which generated the RAPD profile for the three O. vittatus populations were selected. The best amplication using from four primers resulted are OPA 18 in fragments ranging in length 400 - 1300 bp assigned to 22 loci. The study results show that the genetic diversity of the populations Singkarak Lake is 0.1250, Antokan River is 0.0431 and Koto Panjang Reservoir is 0.1512. While pairwise Fst comparison test showed significant differences (p