Identification of green seaweeds Caulerpa scalpelliformis (R.Br.) Weber- van- Bosse and Caulerpa corynephora Montagne using TLC profile

Abstract

The present study was aimed to produce the identification, chemical marker for the two seaweeds viz., Caulerpa scalpelliformis (R.Br.) Weber- van- Bosse and Caulerpa corynephora Montagne using steroid and phenolic TLC profile. Phenolics and steroid profile was detected using the specific mobile phase and spraying agents. The appearance of blue colour and bluish green colour spot in the TLC chromatogram indicated the presence of steroids and phenolics in the crude extracts of C. scalpelliformis and C. corynephora respectively. The ethanolic extracts showed the maximum number of spots. Ethanolic extract of C. scalpelliformis and C. corynephora showed ten and nine steroidal and phenolics bands respectively with different Rf values, Ethanolic extract of C. scalpelliformis and C. corynephora showed ten and nine steroidal bands respectively with different Rf values. The TLC profile developed for the identification of C. scalpelliformis and C. corynephora is simple, precise, specific, accurate, rapid and cost effective and also act as a biochemical marker for these medicinally important seaweeds in the pharmaceutical industry.