The present investigation deals with in vitro Callus induction and plant regeneration of Abutilon indicum (L.)

Abstract

Abutilon indicum (L.) is an important medicinal plant which belongs to the family Malvaceae. It is widely used as folk medicine and treating for many diseases. The efficient in vitro regeneration of Abutilon indicum was achieved from leaf, node and internode explants on MS medium with B5 vitamins and different concentrations and combinations of PGRs like BAP, NAA, IAA, IBA, KIN, 2, 4-D and AdS were tested. The best response for callus induction was observed from leaf, node and Inter nodal explants in combination of BAP (8.88 μM) and NAA (0.53 μM). The high frequency of shoot regeneration was observed from leaf, node and Internodal explants on BAP (8.88 μM), 2, 4-D (0.53 μM) and Ads (0.578 μM). The regenerated shoots were transferred in to half strength MS medium fortified with IBA for shoot induction. Plantlets were successfully acclimatized and transfer in to the field conditions.